Differentiation of Mouse Yolk Sac Cells to Erythroid Cells in The Presence of Erythropoietin
نویسندگان
چکیده مقاله:
Purpose: Yolk sac hematopoietic stem cells (YS-HSC) have two dominant characteristices: a larger reproductive capacity and the absence of the expression of MHC associated antigens. Therefore, these cells are promising candidates for transplantation, cell therapy and gene manipulation. There are controversial reports on the effects of erythropoietin (EPO) on the differentiation of yolk sac cells (YSC) to erythroid lineage in vitro. Materials and Methods: The yolk sacs were dissected from 10 - day - old NMRI mouse embryos and separated mechanically and enzyme digestions (0.1% collagenase or 0.25% trypsin/O.02% EDTA). The colony assay of these cells was performed in the presence of interleukin-3, stem cell factors and different dosages of EPO in a semisolid medium. A control group was also considered in the absence of EOP. After 14 days, benzidine staining on the colonies was carried out and positive benzidine colonies were counted as erythroid colonies. Also, the cytospine slides of those colonies were prepared and stained using benzidine and wright giemsa staining. There was not good result from direct culturing of yolk sac cells. So these cells were co-cultured on the bone marrow stromal cells as a feeder layer. Results: The results showed that in the presence and absence of EPO, the YSCs were differentiated to erythroid cells. However, the number of the positive benzidine colonies (55.4%) and the size of colonies in the presence of EPO (1 U/ml) were increased compared to the control group. There was no statistically significant difference between these groups. Co-culture of these cells with feeder layer also revealed that in the presence of EPO, a better differentiating effect could be observed. Conclusion: In conclusion, in vitro differentiation of YSCs to erythroid lineage was seen in the absence of EPO. However EPO could have a positive effect on the improvement of the erythroid colonies, it needs more investigation.
منابع مشابه
differentiation of p19 embryonic carcinoma cells to erythroid and non erythroid cells in the presence of interleukin 3, 6 and erythropoietin
introduction: setting up a system to study the in vitro hematopoiesis is helpful to understand the mechanism of hematopoiesis. the effects of several cytokines and hormones such as erythropoietin (epo) in differentiation of hematopoietic stem cells have been investigated in vivo. there are no records considering the potential differentiation of p19 cell line (embryonic carcinoma cells) to hemat...
متن کاملThe effects of erythropoietin on haem synthesis in mouse yolk sac and cultured foetal liver cells.
Erythropoiesis in the foetal mouse is reported to occur initially in the yolk sac, followed by the liver, the spleen and finally the bone marrow (Snell, 1956; Borghese, 1959). At 12 days of gestation 97 % of the circulating erythrocytes are nucleated (Craig & Russell, 1964) and presumably arise from the yolk sac blood islands. The cells of this generation persist in decreasing numbers until the...
متن کاملStimulation of haem synthesis by erythropoietin in mouse yolk-sac-stage embryonic cells.
Cultures of disaggregated cells from mouse embryos at the stage of yolk-sac erythropoiesis have been used to test sensitivity of primary erythroid cells to erythropoietin, the hormone which controls adult red cell production. Synthesis of haem was stimulated by 89% in cells of 8-day embryos, 23 % in cells of 9-day embryos and by 19% in peripheral nucleated blood cells of 12-day embryos. Differe...
متن کاملDifferentiation of Mouse Embryonic Stem Cells into Hematopoietic Cells
Purpose: Differentiation of Mouse embryonicstem cells into Hematopoietic cells. Materials and Methods: In this study, we used EB formation system for Hematopoietic differentiation of mouse embryonic stem cell (Royan B1) in suspension culture. EBs cultured in medium with Hematopoietic inducer cytokines (SCF, TPO, GMCSF, IL3, Flt3 and EPO) .presence of hematopoietic differentiated cell assessed ...
متن کاملFunctional Concentrations of BMP4 on Differentiation of Mouse Embryonic Stem Cells to Primordial Germ Cells
متن کامل
Erythropoietin Effects on Fetal Mouse Erythroid Cells
The effect of the hormone, erythropoietin, on cultures of erythroblasts derived from the livers of fetal C57BL/6J mice was examined. An increase both in the content and in the rate of synthesis of normal adult mouse globin chains was detected in hormone-treated cultures. The rate of protein synthesis by individual erythroblasts does not increase in response to the hormone, whereas the absolute ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 2 شماره 1
صفحات 1- 9
تاریخ انتشار 2004-04
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
کلمات کلیدی برای این مقاله ارائه نشده است
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023